Friday, June 29, 2007

Mutating into a Scientist

The torture of my ridiculous 2nd rotation is over. I presented yesterday. I feel like everyone thought I was the worst presenter they'd ever seen. I felt like Dr Beatty was just ashamed, dissapointed. But the truth is that I do not care. I am just glad to be out of there. At the same time this job is so heavy on your emotions and your stress that you even miss its worst moments. It is like a victim missing her abuser or something. So what do I mean by that? Well here I am at home, and instead of being completely happy that is over, on my first day off, my first day that I have overslept, Here I am missing my pipettes, missing experiments missing science.

Why is it that the longer I stay in this program the more obsess I become to science? Why is it that I can't even stay away from it for a day? I can't even bear to think of a whole week? What is going on? Am I mutating into a scientist? Has the mutation begun? Already at such an early stage.

I got to say I've learned hell of A LOT these past few semesters and rotations. When I first got to Berkeley I could hardly distinguish between a Western blot and a Northern and a Southern. I mean I would actually confuse them. Now they are so different to me. Now I know what I am looking for when I look at a gel.

This week off is not even really off. I need to re-read all of my HIV books again. I need to read my new boss's grant. I need to know as much as possible or else.... I want this new boss to want me in his lab. I want to be good. And at the same time, there are just NO excuses, cause baby this is HIV, Immunology, and Isa, we are talking about. I am supposed to know this! And of course I do not want to dissapoint anyone.
I am the first Berkeley student my new boss will experience. I can't afford to look like an idiot to him.
The last 2 presentations that I have done for my rotations can be summarized as pitiful, at least in my opinion. I want my 3rd rotation presentation to be phenominal in MY OPINION. I want to make so that when the professor asks me a question I can answer it and WILL! I don't want the professor to start a whole new conversation with my rotation mentor. I want my presentation to be REALLY GOOD. Impressive! So I am definitely gonna work on it really hard this time. I am not going to let myself get so buried in my work that I don't work on my presentation. That is all... That is a promise to myself.

My current boss, she wants to meet with me next week to talk. I don't know exactly about what. I am going to ask her to be crudely honest. I need the truth, so that next time I can know what I need to do.

Anyways....GOts to go start reading folks... sees you later!

Wednesday, June 20, 2007

Drama and my Political thoughts....

One common denominator that you can gather from all grad students is that they are all hard on themselves. That of course, does not exclude me.

There are many moments in which I look back at undergrad, I look at my GPA and I wonder, what the hell happend. What the heck could have possibly happend that would prevent me from having a better GPA. I go thru my list of excuses, I explain to people what happend and they just look at me with disbelieve. They hardly can believe all the things that happend during highschool and my undergrad. I myself in the mean time feel like I am just going thru a list of excuses that don't measure up. But just this past week something happend that reminded me how it feels to be in a huge family full of extraterrestrial problems.

My stepfather is a legal immigrant. He has been in the United States for over 20 years. He was actually trying to apply for citizenship just last year and he passed everything except the English reading part of the test. He knew about the structure of our government. He knew the history of the United States. He tried testing me on how many members there are in congress, in the house of representative how many years do they last in office....All that stuff. I failed so miserably cause the last time I ever thought about those things was in Junior High Government class. But he knew all the numbers...

He knows how to speak English but he has never really seen the need to learn how to fully read it. He used to work in construction. He was so good at it that he almost started building his own company, but my stepfather is a farmer. He is a simple person. He is not interested in becoming a CEO. All he wants is for the world to leave him alone. All he wants is to get home, see his children, have some drinks with his brothers, and fall asleep to the sound of some Bachata.

My entire family is constantly filled with problems that he runs away from by driving trucks across the country. Thats right, in order to escape my mom's (and other family member's) thirst for conflicts he drives trucks for a living. He goes for days at a time driving back and forth.
Only this time he drove someone else's route into New Mexico. He hit an immigration check point and they asked him for his papers. When he didn't have the papers he told the officer "but I am not going anywhere international"... The officers didn't care they arrested him. The most ironic thing is that his prisoner number is his residency number (National resident of the United States), so they knew he was legal!
It makes my blood boil.

Now they have had him in Jail for a week. There is no bail. My mom is going to have to scrounge up $20,000 DOLLARS out of NO where because the glorious United States Government has my stepfather arrested without charges, without cause, and with proof of his innocence, proof of his legal residence. I don't know about you but this sounds like a GRAND scam to me.
When my mom contacted a lawyer the lawyer adviced her: He should get a better job. He should get a job that keeps him closer to his family. Which is another thing that makes my blood boil. What the FUCK does his job have to do with this? My stepfather is a LEGAL resident you stupid FUCK! Which SHOULD mean that he has the same rights as a LEGAL citizen of the United States. Which SHOULD mean that he can have any job he wants in the WORLD!

I've encounter a lot of people who, when you mention some mistake in the system here in the United States they crucify you. They tell you basically that there is nothing NOTHING wrong with the United States. You tell them, this immigration thing is just not right....the idea of wanting to just throw everyone out, of just arresting everyone who looks a little "Off White".. And they just yell at you, "THEY are all criminals! They are all stupid! They are all evil"....

It is so sad. The United States used to be something really special to me. It used to be the land that saved my family's life (my family ran away from persecution in Dominican Republic during Trujillo's time). One more ironic thing is that the first thing our past (Trujillo) dictator did, is put checkpoints of citizenship all over the country.

In reality it makes my hair stand to see how many new laws in the last 8 years seem very similar to the laws that the Dominican Republic had while under dictatorship. I mean I may not remember how many years is the term of a senator or a representative but the one thing I know is that there were no random immigration check points within the United States (not placed on the border) in the 1970's and I am preaty sure there were none in the 1980's. The US is turning into something ruined, something scary. What most Americans don't understand is that by writing the country off as "PERFECT" and not wanting to rethink, recognize, or even discuss the things that may need changes or correction, they themselves are slowly walking into a path that leads a country into not being FREE, into being just like the land that my family (a generation ago) had to run away from.

Democracy is hard people. You have to know what is going on, you have to look at your opponents point of view. You have to consider consequences years ahead, and alternatives. We are not doing that anymore. Everyone is just considering their own point of view, and in the mean time even LEGAL immigrants are getting hurt, really badly. How badly?
Well here is a simple (almost middle class) family trying to make it and yet my mom is going to have to come up with 20,000 dollars, my stepfather has not worked (so far) in a week.
So what? What does it matter you ask? These are just uneducated simple stupid immigrants you say? Well, you are so wrong... My sister got an offer from Harvard and Yale for her undergrad and here I am.....at UC Berkeley.
What people don't understand is that brilliant people come from simple families too. Simple families are just as valuable as RICH OLD money folks.
So this is just one of the many example of the kinds of things that I have to endure while I am in school.

My sister called me last Friday, hysterical because her father was in jail. She'd never known anyone in jail because all the other drama happend years ago when she was too little to remember. These kinds of things, legal, emotional, physical, international problems are very common in my family. I had to calm her down....

I saw a quote the other day:
They came for the Jews
But I am not Jew so I didn't do anything about it
They came for the Homosexuals
But I am not gay so I didn't do anything about it
Then they came for the Black people
But I am not Black so I didn't do anything about it
Then they came for ME
But there was no one to defend me cause everyone else was already GONE!

Also there was a quote I saw one day in a New York restaurant bathroom which I think also applies to the state of our democracy right now.....
Jazz is not dead
It just smells funny...

Replace the word Jazz with American democracy please. This is definitely smelling funny to me....
SO, What do you think? Do you think that if all the sudden you found out your parents were in jail that would affect your grade on a test the next week? I think so.

Sunday, June 10, 2007

Lab Presentations Suck...Specially in Labs that you Don't Like

Yeah, so my lab presentation is in 2 weeks. I know, why am I stressing about it now? Well I am stressing cause I haven't read the background on this project, Not enough to make a lab presentation.
So here I am feeling to lazy to read and yet in the back of my mind, worrying.
Do I even have some results? Well I have a few things. And also a few more experiments that I've set up and will set up this week before the weekend.
Carlos' Birthday is this week (Thursday). How the hell am I going to do everything I need to do is a universal mystery. Thats right, Aliens 3 galaxies away from this one are watching us with a special microscope and they are wondering how I am going to get away with this one.
Another bad thing is that the Gringa from HELL is going to be there. Actually there are a few gringas from hell in this lab but the most evil will be there.
I am not afraid though, I've faced the devil many times. This witch can just kiss my ass!
Anyways it is 10:30 pm on a Sunday night and I am, ofcourse, in lab where are you at this onGOdLY hour? Do you know where your kids are?

I am rambling cause I am hungry and bored to DEATH. Have I mentioned how much I hate this lab?

Thursday, June 07, 2007

We are going to UCSF

Yep!
I got a rotation in UCSF. This is a lab that seems to have a lot of money. The lab is brand new. The PI is really well recognized, I think he is head of the program there or something so big time!
AND WE ARE DOING HIV!
YEY! Honey I 'm home!
I have to respond. I have to choose a project! He has so MANY! I have to start reading. I have to do good in this lab. There are NO EXCUSES here I am supposed to know HIV up and down. In this lab you have to show results every week! So some how I have to stop being burnt out between today and July 10th when I start!
OH my GOD I am so scared, excited, happy did I mention SCARED?!
HIV YEY HIV YEY HIV YEY HIV YEY HIV YEY HIV YEY!

Saturday, June 02, 2007

To be me is to be....

To be me is to be...
Is to be thinking of 3 different things at the same time,
It is to be angry and yet a little happy and then again depressed,
It is to think you are no better than trash but then again you are so proud of all the dumpsters you've been in.
To be me is to NOT deserve it not even a little.
To be me is to be worrying even while you are dreaming,
it is to always be fighting against everyone and everything,
And to never look back, not even at the things you've accomplished.
What do I mean?
Ofcourse I know the things I've done. But I never think any of them comes up close to being enough.
To be me is to be waiting for the next problem,
To expect people to dissapoint you,
To be me is to have 1000 times the energy compared to anyone else
Enough to hate everyone and everything
Enough to love the people around you like if they were your own children like if they belonged to you.. Don't forget, this is all at the same time.
No self-pity now, that is only for the weak and besides...Millions of other people have had 100x's worse lives than I.
To be me is to obsess over everything and anything because you don't know any other way to survive and you are afraid that life, like a house of cards, might just crash if you change a thing.
Constant fear of failure yet a tendency to want to destroy it all.

--------------O-------------
Isa placed her head on Carlos' chest while they sat on their big giant couch filled with shaggy pillows. They had just finished eating breakfast and there was a commercial on TV.

Isa: I am so glad it is over. That horrible time of my life.
Carlos: It never happend... You were sleeping.
Isa: A bad dream? A nightmare, thats what it was?
Carlos: Absolutely...
What would I do without him-She thought.
Isa: You are my buffer.
Carlos: You make me sound so boring.
Isa: No. You keep me steady. You keep me from destroying everything. I am acid and you are my buffer.
Carlos smiled.

God it is a miracle that I am here. All of this.. This apartment, these books, this TV, someone that loves me. And then there is school. I am a graduate student. I am supposed to be in Washigton Heights prostituting, using drugs, or at least pregnant with my 3rd child from different husbands, with the current husband being a drug addict and beating me up. Thats what I am supposed to be. Thats what statistics say, thats what family history says and yet...I am here. Right here. Maybe thats why I never liked Statistics class. Cause they are never right....with me.

Friday, June 01, 2007

IT WORKED!

Okay so usually when I use this blog to say that something worked, the shit starts hitting the fan again. So I am getting to the point where I think that it is bad luck to say that something worked on this blog, but right now I can not help myself. I am too excited. So here it goes: IT WORKED!
Yeah, there you go. Now if bad stuff starts to happen again, the return of the evil green gnomes, I will never again tell you guys my results online.
The story of the procedure that worked is that there was this FACS I had to do, and I was basically left to do it all on my own. I only got 10 uL of antibody to stain with and although I did mess up a few things in the experiment I still got results! It was soooo exciting. There I was sitting in the Flow cytometry facility and getting results. I was so happy. You don't understand I was so worried about this experiment not working that I woke up in the middle of the night like 5 times wondering if my cells were okay. I was so worried I was acting like a psycho this morning. Sheri was laughing at me, Diana was asking me when I was going to take a vacation... Karen and Shanty avoided talking to me.
So when I came back from the FACS facility I was SOOO excited that I couldn't help it. I had to tell someone and I thought that since Jennifer had helped me that I should let her know that it worked. But while I was telling her, she just looked like she couldn't wait for me to shut up. So then I went to Old Frows lab, and there I just screamed it out.
hehehe
I love FACS. hmmm I should make a T-shirt off of that one.
Now my friends from Albert Einstein are going to find this one hilarious. When I was at AE, I would always use the program Cell Quest Pro to acquire and analyze my data. People would tell me to use Flow Jo and I always rejected it because I always thought that it was sooooooo complicated and unnecessary since I was an expert at Cell Quest. Now there is absolutely NO Cell Quest at Berkeley. All they have is Flow Jo so my data is going to look like SHIT until I decide that it is okay and that I have to use Flow Jo.
So there, you guys won, you were right, I should have learned Flow Jo so that today my data would have been PERFECT!
I don't know though, if it is actually worth it for me to learn it yet cause I don't know if Jose is going to let me do the experiment again. And I already know that if I start working in UCSF they have other programs. I really like this part of the project I am kind of getting attached to it eventhough I am leaving the lab at the end of June.

Hey! We are going to TAHOE!!!! I am taking at least 1 fiction book to Tahoe so that I can cheat on science...for once.

So I see you guys are commenting again. I am glad your fingers are functioning again. HAHHAHAHA! Glad you guys are commenting and reading.