Friday, June 01, 2007

IT WORKED!

Okay so usually when I use this blog to say that something worked, the shit starts hitting the fan again. So I am getting to the point where I think that it is bad luck to say that something worked on this blog, but right now I can not help myself. I am too excited. So here it goes: IT WORKED!
Yeah, there you go. Now if bad stuff starts to happen again, the return of the evil green gnomes, I will never again tell you guys my results online.
The story of the procedure that worked is that there was this FACS I had to do, and I was basically left to do it all on my own. I only got 10 uL of antibody to stain with and although I did mess up a few things in the experiment I still got results! It was soooo exciting. There I was sitting in the Flow cytometry facility and getting results. I was so happy. You don't understand I was so worried about this experiment not working that I woke up in the middle of the night like 5 times wondering if my cells were okay. I was so worried I was acting like a psycho this morning. Sheri was laughing at me, Diana was asking me when I was going to take a vacation... Karen and Shanty avoided talking to me.
So when I came back from the FACS facility I was SOOO excited that I couldn't help it. I had to tell someone and I thought that since Jennifer had helped me that I should let her know that it worked. But while I was telling her, she just looked like she couldn't wait for me to shut up. So then I went to Old Frows lab, and there I just screamed it out.
hehehe
I love FACS. hmmm I should make a T-shirt off of that one.
Now my friends from Albert Einstein are going to find this one hilarious. When I was at AE, I would always use the program Cell Quest Pro to acquire and analyze my data. People would tell me to use Flow Jo and I always rejected it because I always thought that it was sooooooo complicated and unnecessary since I was an expert at Cell Quest. Now there is absolutely NO Cell Quest at Berkeley. All they have is Flow Jo so my data is going to look like SHIT until I decide that it is okay and that I have to use Flow Jo.
So there, you guys won, you were right, I should have learned Flow Jo so that today my data would have been PERFECT!
I don't know though, if it is actually worth it for me to learn it yet cause I don't know if Jose is going to let me do the experiment again. And I already know that if I start working in UCSF they have other programs. I really like this part of the project I am kind of getting attached to it eventhough I am leaving the lab at the end of June.

Hey! We are going to TAHOE!!!! I am taking at least 1 fiction book to Tahoe so that I can cheat on science...for once.

So I see you guys are commenting again. I am glad your fingers are functioning again. HAHHAHAHA! Glad you guys are commenting and reading.

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