Friday, February 16, 2007

Just one of those weeks...

Today I realized I ordered the wrong primers, my IFA looked like crap, I didn't add antibiotics to my Broth, many of my cells are getting contaminated for no reason. That was $40 per primer. That was 4 days of work on the IFA. And for the broth, I had the perfect amount of bacteria... :(
SOOOOOO many things went wrong today.
Rick was nice about it. He told me not to let it bother me. Sometimes you just can't do anything in the lab.
The one thing that worries me the most is that I've got no reason to be burnt out. There is just sooo much I need to do. I need to study for Infectious Diseases, I need to work on my paper for Virology. I need to read for lab, cause I have officially forgotten why am I do anything and my lab presentation is coming up REALLY SOON!

I met with my professor today. He asked me on the progress of the project. Then he told me I should start my HIV project on 3-12-07. Thats 1 week before my presentation. What in the world am I going to do? He doesn't know either. Infect some cells I guess. I should be exciliratingly happy but he has already basically ruined it for me. I dont trust him. I asked him about getting some vaginal mucosal cells, since the virus in the blood doesn't represent the vaginal mucosa at all! He said to give him a chance to put everythiing in order and then we would see. Makes sense, if I am not going to stay here we wouldn't want to oder expensive cell lines...
It is friday. I wish i could take a day off so that I can calm down about all this, study for ID and start all over again.
That reminds me of that song: I pick myself up, Rub it myself off, and start all over again....
Thats what I need to do.
I yelled at Ed today... He asked me what had happend with the PCR. I kept telling him not to ask about the details. He kept asking so my voice went very intense.
I apologized 5 minutes later, when I realized why everyone had been staring at me. I felt so bad. He said he didn't feel bad at all cause he already knows me. He already knows when I am pist off at him or at the PCR and he was sure all along I was pissed off at the PCR.
They are sooooo sweet to me in this lab.
I am going to miss them.

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