Well, I went to class, I did a presentation, then I went to work, I attended lab meeting. While lab meeting was going on I was in the middle of a large prep so everytime someone finished their presentation I had to go do some stupid step in my large prep.
I needed this DNA for today so I was very enthustiastic.
SO finally I got to the part where you run your DNA thru your column. Check, well done. But the next step where I am supposed to add Isopropanol to the DNA so that it will precipitated while centrifuging I fucked up. Instead of adding isopropanol I added ethanol! Yes my friends your beautiful lovely friend Isa fucked up majorly. So I called Maria. I called Sheri. I called Quiagen. I looked in the internet. Apperently no one in the world had ever done such a stupid thing in their life cause no one knew what to do.
I swear to GOD I was "Dead en el Piso"!
So I saw the precipitate but according to the website that white stuff you see is not your DNA it is SALT. So I was scared to death. Sheri said that it shouldn't make such a difference since EtOH is not that different from Isopropanol and either way some DNA extractions are done with EtOH. I had thought the same thing but I didn't believe it. I prayed to God. But I am not exagerating I heard him say in my head, "NOw you want me to turn EtOH into PropanoL? Now you want me to change chemicals? You know there is only so much I can do about your FUCK UPS." I begin to doubt everything. Why am I trying to become a scientist? I suck. But then I remembered that in less than a month I have done 3 perfectly beautiful presentations! Conflict conflict conflict. I remember that while I was grinding my teeth none of my experiments failed. Maybe grinding my teeth and destroying them and getting migraine headaches is worth me not doing mistakes?
Dead en el piso. Thats what I was.
THEN I specked the samples anyways... And the Angels sung. Ahhhhh!
I got DNA!!!!!!!!!!
YEY
I got DNA. Watson and Crick were not as happy as I was
(I know this for a fact because they were Stiff British thieves).
Dead en el piso. Thats what I was.
THEN I specked the samples anyways... And the Angels sung. Ahhhhh!
I got DNA!!!!!!!!!!
YEY
I got DNA. Watson and Crick were not as happy as I was
(I know this for a fact because they were Stiff British thieves).
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