Too tired to be tired

So this friday I was dying cause I am still not sure if my transfections worked. You see the way things work is that I have to test out my viruses. I have to test them out on cellular entry under different conditions. In order to do all these experiments obviously I need virus. My next presentation is on the 25th of October.
If I don't have virus by this week then I can't do experiments. If I can't do experiments then can't have results for the presentation. Now I want to have results for this presentation because on my first, I didn't really have any. Most of my presentation was about making viruses and the troubles we were having with it.

What scares me about my transfections, what could have been wrong this time? Well there is the DNA which I precipitated with EtOH instead of iso-propanol. Then there is the cells. I discovered last week that one HUGE problem is that the shelves in the incubator are NOT leveled. So basically what ends up happening is that my cells accumulate on one side of the flask and then those become too crowded while the other side is not so crowded. They are supposed to be evenly distributed. I guess one thing that I can do is leave it in 1 position for 2 hours and then turn it the opposite way for another 2 hours..... Maybe they'll be even then?

In the mean time, in this transfection, I had bad DNA (as far as I am concerned) and then I also had uneven cells. Also I waited 1 hour before putting the precipitate to the cells. But it took me a long time to prepare the cells after that one hour so I might have left the precipitate growing for too long. I noticed a lot of flakes once I added it.

Sorry kitties, I am trying to write all the possible failures here.
I am going to run an ELISA on these today so that I can see if I have virus or NOT. Wish me luck.

In the meantime, next week monday is my first immunology test and "Frankly my dear, I don't give a damn." Thats the attitude I have right now and I can't shake it off. I played video games with Carlos for 3 hours last night. Thats how much I don't give a crap about this test.