Saturday, May 10, 2008

happy times and fucked up times- In Lab

hi kitties,

So I went to the Institutes' retreat and had fun. For a minute there I thought that everyone was fun and not that bad at all. Then all the sudden yesterday I get a blood sample. I wanted to isolate my PBL's which was a successful procedure. Then I had to activate my T cells with IL2. This is where I failed.
So there is supposed to be this lab stock of IL2. A post doc in the lab prepares that stock and people take some whenever they needed. I didn't think of asking people for IL2 or anything cause I was depending on the stock. Then when I went to the stock box I found nothing in it. NADA. This is at 8:30 pm when I was trying to make it to Maria's for an emergency Fight Club meeting. No one else was in the lab that knew anything about IL2.
I went and checked the freezer again, checked all over the place. Every box every shelf. NADA. So then all the sudden I see this other box that indicates IL2. In the box I find this eppindorf with the alpha sign but it had the same concentration as IL2. Maybe it is a 2 and not an alpha. So I call Dave. Dave says go ahead use it, it must be IL2. Well, I added I finally picked up the phone to call the girls and instead there was a messege saying that the fight club meeting had ended. I call Maria, she doesn't answer, classic.... I call Sheri we talk for a while about recent events and the mystery of graduate life. I go home I come back the next day only to find out that what I had thought was IL2 was actually IFN alpha. I realize that all of my cells have to be thrown out.
I couldn't help get pist I mean real pist. If the lab stock would have been where it should've been then this would not happen. It is not like I can get PBL's this week again or next week. This means everything is delayed by 3 weeks. I got so pist that I ended up having a tiff. No I didn't scream, I didn't yell. I ended up venting to some one about how if you are responsible for stocks you should be responsible and put them where they are supposed to be. Well instead of saying you need to come down it is not such a big deal. Mistakes were made. I'm sorry your cells are shit now. Instead of any of that I get the, you are acting psychotic, I don't like your tone, why are you coming on so strong, I don't know what you are talking about, why don't you take 2 days off, talk.

Yeah it was great. Not only did I feel like people were hiding stocks from me but now I felt like I was being thrown out of the lab for complaining about stocks. GOD FORBID I ever complain about anything.
I have lab meeting in June and according to one of the lab people, I need to prove myself in this lab meeting or I am toast. We might as well throw away the toaster cause I am soooo burnt.

I wanna quit this lab so badly, so so so so badly. Hell, I want to quit Grad.

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